Coding

Part:BBa_M36227:Design

Designed by: Akshay Rajagopal   Group: Stanford BIOE44 - S11   (2015-10-24)

Modified CAD1 from A. Thaliana (S. Cerevisiae optimized)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In this part, the CAD1 gene has been stripped of all introns in order to save space and resources. Furthermore, the sequence was optimized for expression in S. cerevisiae cells based on prevalent codons. This was performed using IDT's codon optimization tool.

Source

The original source was the CAD1 gene found in A. Thaliana.

References

1. Cobbet C. 2000. Phytochelatins and Their Roles in Heavy Metal Detoxification. Am Soc of Plant Phys. 123(3):825-832.
2. Ha SB, et al.1999. Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell. 11(6):1153-64.
3. Vatamaniuk OK , Mari S, Lu Y, and Rea PA. (2000). Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase. J Biol Chem. 275 (40): 31451–3145900.